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| Sequi-Gen GT |
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The patented Sequi-Gen GT nucleic acid sequencing cell takes the trouble and frustration out of casting and running sequencing gels.The unique Sequi-Gen GT cell ensures smile-free patterns, and has IEC 1010 safety certification.In addition, the Sequi-Gen GT cell introduces an easy horizontal casting method that dramatically improves gel casting.
PowerPac HV recommended (see Electrophoresis Power Supplies). Simple Horizontal Gel Casting Method Assembling and casting with the Sequi-Gen GT cell is convenient and reliable. The lever clamps slide over the gel sandwich. Each clamp exerts an even pressure over the entire length of the glass plate sandwich, resulting in a tight seal. This prevents damage to the plates or leakage which can result from uneven pressure. Tape, screws, and grease are unnecessary. With the new casting method, a precision caster is attached to the bottom of the Sequi-Gen GT cell. This assembly is placed flat on the lab bench, and the gel is injected with a syringe through an injection port in the precision caster. Gel casting does not get simpler.
Unique Design Eliminates Mobility Artifacts The Sequi-Gen GT nucleic acid sequencing cell incorporates a unique upper buffer chamber, called an integral plate chamber (IPC). The IPC is a glass plate bonded to an upper buffer chamber with a silicone adhesive. This forms a leak-free, thin upper buffer layer that covers the entire area of the gel plate. The upper buffer acts as a heat sink that maintains uniform heat distribution over the entire gel area during electrophoresis. Uniform heating prevents sample mobility artifacts, such as smiling, and greatly reduces cracking of plates. The assembled IPC offers electrical safety, unlike traditional aluminum plate heat sinks.
Integral plate chamber. The upper buffer chamber extends over the entire area of the bonded gel plate, contacting the top of the gel near the fill spout.
Uniform heat distribution eliminates smiling artifacts. Reactions may be loaded across the entire width of the comb, increasing sample throughput. Four Interchangeable Sizes Fit all Applications There are four different IPC sizes for the Sequi-Gen GT cell. The space-saving universal base acts as the lower buffer chamber and can be used with all four IPC sizes. The newest 38 x 30 cm size is wide enough to load 100 samples, but short enough for applications that do not require long run times, such as microsatellite mapping, SSCP, and oligonucleotide analysis.
Four interchangeable Sequi-Gen GT sizes. Sequi-Gen GT Laboratory Applications —Not Just for Sequencing Anymore The long, thin, polyacrylamide gel format of the Sequi-Gen GT cell provides excellent separation of DNA and RNA fragments that differ by only a single nucleotide. In addition to DNA sequencing, this feature is useful for* Differential display * Microsatellite analysis * DNA fingerprinting * Single-Strand Conformation Polymorphism (SSCP) studies * Heteroduplex analysis * DNA footprinting * RNase protection assays * S1 nuclease mapping * Primer extension analysis * DNA/protein binding studies (gel shift assays) * Oligonucleotide analysis
Microsatellite analysis. Photo contributed by Catriona MacGeoch, David P. Kelsell and Nigel K. Spurr, Imperial Cancer Research Fund, UK.
When performing these applications in conjunction with the PowerPac HV power supply with temperature probe, constant-power, -voltage, or -current electrophoresis is possible. The PowerPac HV power supply also offers ultimate user safety with arc detection, ground leak detection, and no-load detection features. Sequi-Gen GT systems can be ordered with or without the economical, IEC 1010-1 safety certified, PowerPac HV power supply with temperature probe. Specifications
* U.S. patent number 4,663,015
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